The stem part of Tinospora cordifolia(Wild.) Hook. F. And Thomas of the family Menispermaceae were collected from the Saptari district of Nepal. The taxonomic identification was carried out by Prof. Dr. Shiva Kumar Rai taxonomist of Post graduate college Biratnagar Tribhuvan University department of botany.Plant material were collected in the month of May climbing on the mango tree.
From the collected plant materials stems were separed and washed, dried in shade and crushed to coarse powder.
3.2 Preparation of Extract
The plant stem were washed thoroughly with tap water followed by sterile distilled water and shade dried at room temperature for 10-15 days.Then for complete dry the plant material were kept in oven at 45? overnight.